The effect of streptozotocin diabetes on the levels of glycolate and lactate excreted in rat urine. |
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Authors: | R P Metzger R D Sauerheber S A Lyons J R Westall |
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Institution: | Departments of Physical Science and Chemistry, San Diego State University, San Diego, California 92182 USA |
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Abstract: | The concentrations of glycolate (hydroxyacetate) and lactate are significantly elevated above control values in urines from streptozotocin-diabetic rats, regardless of whether data are expressed in terms of μg/ml urine or μg/day. The same levels of oxalate and glyoxylate are excreted in 24 h in the urines from normal and diabetic rats. Lactate levels are elevated above control values in serum from streptozotocin-diabetic rats.The elevation of glycolate levels in diabetic rat urine compared to control values occurs regardless of diet and regardless of whether rats were fed or fasted during the 24 h urine collection period.Rat liver glycolate oxidase may be used to assay glycolate concentrations in the presence of up to 500 μg/ml l-lactate when pH 8.6 Tris-Cl is used as buffer. Results obtained with this assay compare qualitatively with the standard colorimetric assay using 2,7-dihydroxynaphthlene for glycolate determination. Beef liver glycolate oxidase is not effective for use in glycolate assays. The identity of urinary glycolate was confirmed by gas-liquid and by paper chromatography. |
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