Sunflower Nitrate Reductase: Purification and Characterization |
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| Authors: | Debasis Pattanayak Sukumar R Chatterjee |
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| Institution: | 1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India
2. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110 012, India
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| Abstract: | A single isoform, NADH: nitrate reductase (NR), was purified 500 folds from sunflower leaves by affinity chromatography on Blue Sepharose CL-6B. Purified NR had a pH optima of 7.25 and a molecular weight of 210 kD. In SDS-PAGE, two bands of 47 and 56 kD were obtained. NADH: ferric citrate reductase activity was copurified with NR with a specific activity of 2. The Vmax of NADH: ferric citrate reductase was 8.69 units mg-1 protein and the apparent Km for ferric citrate was 0.435 mM. |
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