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Employing a Crude Toxin Preparation from Sarocladium oryzae as a Molecular Sieve to Select Sheath Rot-resistant Somaclones of Rice
Authors:R. G. Hemalatha  S. Jebaraj  J. A. J. Raja  T. Raguchander  A. Ramanathan  R. Samiyappan  P. Balasubramanian
Affiliation:1. Department of Agricultural Botany, Agricultural College and Research Institute, Madurai, India
3. Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India
2. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India
Abstract:This study was undertaken to explore the possibilities of using in vitro techniques for selection of sheath rot-resistant soma clones of rice cv Co43. Oryza nivara and O. longistaminata possessing sheath rot-resistance were included in the study, with a view to comparing the reaction of the susceptible somaclones of O. Sativa cv Co43 to a crude toxin preparation from culture filtrate of the pathogenic fungus, Sarocladium oryzae. A few somaclones of Co43, selecfed on the crude toxin preparation, regenerated into plantlets and their reaction to the toxin was reconfirmed up to R1 generation.
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