A set of procedures for resolving purine compounds by reversed-phase high performance liquid chromatography: application to the study of purine nucleotide and nucleic acid metabolism |
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| Authors: | P Rotllán A Liras P Llorente |
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| Affiliation: | 2. School of Nursing, Guangzhou Medical University, Guangzhou 510030, China;3. Department of Nursing, Children''s Hospital of Anhui Medical University, Heifei, 230000, China;4. Clinical College of The Second Shenzhen Hospital, Anhui Medical University, Shenzhen, 518035, China;5. Department of Neurology, Shenzhen Second People''s Hospital/the First Affiliated Hospital of Shenzhen University, 3002 Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China;1. Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, China;2. Department of Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China;3. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China;2. Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China;3. Tianjin Fisheries Research Institute, Tianjin 300221, PR China |
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| Abstract: | A set of simple procedures for the separation of major purine 5'-ribonucleotides including diguanosine polyphosphates, purine and pyrimidine bases, and 2'- and 3'-nucleotide monophosphates using reversed-phase high-performance liquid chromatography and isocratic elution study of purine nucleotide and nucleic acid biosynthesis in Artemia is presented. |
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