Different individual immune responses elicited by in vitro immunization |
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Authors: | Makiko Yamashita Yoshinori Katakura Sun-Yup Shim Shin-ei Matsumoto Takashi Tamura Kazuko Morihara Yoshihiro Aiba Kiichiro Teruya Toshie Tsuchiya Sanetaka Shirahata |
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Institution: | (1) Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan;(2) Division of Medical Devices, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan |
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Abstract: | We have previously demonstrated that the addition of muramyl dipeptide (MDP), interleukin-2 (IL-2) and IL-4 effectively raises
antibody production from L-Leucyl-L-leucine methyl ester (LLME)-treated human peripheral blood lymphocytes (PBLs) against
specific soluble antigen when immunized in vitro. However, PBLs from individual donors were separate optimal conditions regarding concentrations for IL-2 and IL-4, which
in turn required us to optimize each individual PBLs to effectively produce antigen specific human antibody by in vitro immunization. These individual differences in the requirement for IL-2 and IL-4 reflects the differences in individual immune
responses against a specific soluble antigen, which can be elicited by in vitro immunization. In the present study, we investigated these individual differences in the requirement for IL-2 and IL-4 to
induce antibody productionin vitro in the PBLs of 12 volunteers (9 healthy donors and 3 allergenic patients). IL-2 requirements for antibody production varied
dependent upon each donor, while higher amounts of IL-4 inhibited IgM and IgG production in all of the healthy donors. However,
some of the characteristic features for PBLs donated from allergenic included lowered IgM production compared to PBLs derived
from healthy donors, and very high IgE production in the absence of cytokines and allergen. These results demonstrate that
the sensitivity of PBLs against antigen sensitization differs between healthy donors and atopic patients, which suggests that
the frequency of antigen sensitization might be reflected in differing activation states and/or differing subpopulations of
lymphocytes in vivo.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | human antibody interleukin-2 interleukin-4 in vitro immunization |
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