下调烟粉虱MED隐种BtabCSP6表达对番茄黄化曲叶病毒传播的影响

【目的】番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV)是对农业生产造成威胁的主要病毒之一，自然条件下通过媒介昆虫烟粉虱Bemisia tabaci传播。已有研究表明烟粉虱雌成虫比雄成虫具有更强的获毒与传毒能力。本研究旨在探明烟粉虱化学感受蛋白(chemosensory protein, CSP)基因BtabCSP6表达对病毒传播的影响，为控制病毒发生寻找新途径。【方法】使用TYLCV侵染性克隆方法获得带毒番茄植株，微虫笼收集不带毒烟粉虱MED隐种成虫固定在感染TYLCV的番茄植株叶片获毒48 h;利用RT-qPCR技术测定分别取食感染和未感染TYLCV番茄植株的烟粉虱MED隐种雌雄成虫体内BtabCSP1-8基因表达量变化；通过饲喂法利用RNAi对烟粉虱MED隐种雌成虫BtabCSP6基因进行干扰48 h后饲喂TYLCV感染的番茄植株，测定烟粉虱MED隐种雌成虫的获毒率和传毒率。【结果】RT-qPCR检测结果表明，与未侵染的烟粉虱MED隐种雌成虫相比，侵染TYLCV的雌成虫体内BtabCSP3和BtabCSP6基因的表达量变化最为显著。同样...

Influence of down-regulating the expression ofBtabCSP6 inBemisia tabaciMED (Hemiptera: Aleyrodidae) on the transmission ofTomato yellow leaf curl virus

【Aim】 Tomato yellow leaf curl virus (TYLCV) is one of the main viruses that threaten agricultural production and transmitted by the insect vector Bemisia tabaci under natural conditions. Studies have shown that female adults of B. tabaci have stronger ability to acquire and transmit virus than male adults. The objective of this study is to investigate the influence of the expression of the chemosensory protein (CSP) gene BtabCSP6 in B. tabaci on virus transmission, and to find a new way to control the occurrence of virus. 【Methods】 Infected tomato plants were obtained by the TYLCV infectious clone, and uninfected B. tabaci MED adults were collected in micro-cages and fixed on leaves of the TYLCV-infected tomato plants for 48 h. RT-qPCR technology was used to measure the changes in the expression levels of BtabCSP1-8 in female and male adults of B. tabaci MED fed with TYLCV-infected and uninfected tomato plants, respectively. The BtabCSP6 gene in female adults of B. tabaci MED was suppressed by RNAi for 48 h through feeding method followed by feeding with TYLCV-infected tomato plants, and the virus acquisition and transmission rates of female adults of B. tabaci MED were determined. 【Results】The RT-qPCR results showed that the expression levels of BtabCSP3 and BtabCSP6 in TYLCV-infected female adults of B. tabaci MED changed most significantly as compared to those in the uninfected female adults. Similarly, the expression levels of BtabCSP4 and BtabCSP6 in TYLCV-infected male adults of B. tabaci MED changed most significantly as compared to those in the uninfected male adults. After being fed with dsBtabCSP6 for 48 h, the expression level of BtabCSP6 in female adults of B. tabaci MED decreased. The virus acquisition rates of female adults of B. tabaci MED after feeding with TYLCV-infected tomato plants for different time and the virus transmission rates of female adults of B. tabaci MED in different numbers to uninfected tomato plants were significantly decreased as compared to those of the control. 【Conclusion】 Down-regulating the expression of BtabCSP6 in female adults of B. tabaci MED can significantly reduce their virus acquisition and virus transmission rates, suggesting that BtabCSP6 may affect the transmission of TYLCV.