Autolysis of Porphyromonas gingivalis is accompanied by an increase in several periodontal pathogenic factors in the supernatant |
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Authors: | Kamaguchi Arihide Nakano Miyo Shoji Mikio Nakamura Reiko Sagane Yoshimasa Okamoto Masaaki Watanabe Toshihiro Ohyama Tohru Ohta Michio Nakayama Koji |
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Institution: | Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan. kamaguti@hoku-iryo-u.ac.jp |
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Abstract: | Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two-dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg-gingipain (Rgp) as determined by N-terminal amino acid sequencing. Corresponding to the increase in spot volume, Rgp activity also increased during autolysis. The results of this study suggested that Rgp and other proteins in the P. gingivalis autolysate may be involved with the prolongation of periodontal disease, even after the death of P. gingivalis cells. |
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Keywords: | porphyromonas gingivalis autolysis RGP two‐dimensional analysis |
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