In vitro genotoxic effects of the anticancer drug gemcitabine in human lymphocytes |
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Authors: | Aydemir Nilüfer Celikler Serap Bilaloğlu Rahmi |
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Affiliation: | Uludag University, Faculty of Arts and Sciences, Department of Biology, Gorukle, Bursa 16059, Turkey. aydemirn@uludag.edu.tr |
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Abstract: | This research was carried out to investigate in vitro genotoxic effects of the anticancer agent gemcitabine on the induction of chromosomal aberrations and sister-chromatid exchange in human lymphocytes. Three doses of gemcitabine (0.001, 0.002 and 0.004 microg/ml) were applied to lymphocyte cultures from 15 donors. There was a significant increase in the induction of chromosome aberrations and in the occurrence of sister-chromatid exchange in these cells. In addition, gemcitabine significantly decreased the mitotic index and replicative index for all doses. Dose-response regression lines were used to compare the individual susceptibilities to gemcitabine with respect to the chromosome aberration and sister-chromatid exchange frequencies. Our results indicate that gemcitabine is able to induce both cytotoxic and genotoxic effects in human lymphocyte cultures in vitro in a dose-dependent manner. |
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