| 缺血-再灌注时大鼠心脏Gi蛋白α亚基的变化 |
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| 作者姓名： | Wang Y Wu LL Ge MZ |
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| 作者单位： | 北京医科大学病理生理教研室,北京,100083 |
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| 基金项目： | 国家自然科学基金!No．39570301,教委博士点基金!No.9501014 |
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| 摘 要： | 本工作研究了心肌缺血－再灌注时,受体－腺苷酸环化酶细胞膜信号转导系统中Ｇ蛋白含量及功能的变化。采用免疫印迹法和放射免疫法分别测定大鼠心脏Ｇｉα２,Ｇｉα３和Ｇｓα的含量及腺苷酸环化酶的活性。结果显示缺血－再灌注时心功能明显损伤;心脏Ｇｓα无明显变化;心肌细胞膜Ｃｉα２和Ｇｉα３含量分别升高３７．４％（Ｐ〈０．０１）和４２．４％（Ｐ〈０．０１）;胞浆内Ｇｉα２和Ｇｉα３含量无明显变化;缺血心肌腺苷酸
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| 关 键 词： | G蛋白 缺血－再灌注 信号转导 细胞膜 |
| 修稿时间： | 1997年9月29日 |
Alterations of cardiac Gi protein alpha subunits during ischemia-reperfusion in rats |
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| Wang Y,Wu LL,Ge MZ.Alterations of cardiac Gi protein alpha subunits during ischemia-reperfusion in rats[J].Acta Physiologica Sinica,1998,50(5):514-518. |
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| Authors: | Wang Y Wu L L Ge M Z |
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| Institution: | Department of Pathophysiology, Beijing Medical University, Beijing 100083. |
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| Abstract: | Alteration of G proteins in rat heart during ischemia-reperfusion was studied. Levelsof Cia2, Gia3 and Gsa in sarcolemma and light vesicle were measurdd by Westem blotanalysis. Adenyl cyclase activities were determined by radioimmunoassay. Duringischemia-reperfusion, cardiac function parameters were decreased significantly.Expressions of Gsa remained unchanged. Compared with the control group, levels of Gia2and Gia3 in sarcolemma in ischemia-reperfusion group increased by 37 .4% (P<0.01)and 42.4% (P<0.01), respectively with, however, no changes in light vesicle;Activities of adenyl cyclase decreased during ischemia-reperusion. The results suggestedthat the increased expression of cardiac Gi may contribute to the dysfunction of adenylcyclase signal transduction system and the impaired cardiac function during ischemiareperfusion. |
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| Keywords: | G protein ischemia-reperfusion signal transduction adenyl cyclase |
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