The Antigen Receptor (NCCRP-1) on Catfish and Zebrafish Nonspecific Cytotoxic Cells Belongs to a New Gene Family Characterized by an F-Box-Associated Domain |
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Authors: | Liliana Jaso-Friedmann David S Peterson Daniel S Gonzalez Donald L Evans |
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Institution: | (1) Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7386, USA, US |
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Abstract: | The catfish nonspecific cytotoxic cell receptor protein (NCCRP-1) provides an important function in target cell recognition
and activation of cytotoxicity. This report identifies and characterizes a zebrafish orthologue of the catfish NCCRP-1. The
zebrafish NCCRP-1 cDNA contains an open reading frame that encodes a predicted protein of 237 amino acids with a MW of 27
kDa and a pI of 5.5. Sequence similarities comparisons show that the NCCRP-1 receptors from these two phylogenetically distant species
share a high degree of identity. These results suggested that NCCRP-1 performs a crucial function in innate immunity in teleosts.
Further, a zebrafish 17-mer peptide corresponding to the catfish NCCRP-1 antigen-binding domain inhibited (catfish) cytotoxicity
toward conventional tumor target cells (HL-60). These data appeared to indicate that the zebrafish NCCRP-1 protein may function
as an antigen recognition molecule and, as such, may participate in innate immunity in teleosts. A homology search of the
zebrafish NCCRP-1 protein revealed that it shares a significant level of identity with another group of proteins belonging
to an F-box subfamily. These proteins share an F-box domain in the N terminus (not present in NCCRP-1) and an extremely conserved
C-terminal region that has been termed the F-box-associated domain (FBA). The FBA is currently of unknown function. A new
gene family is proposed in this work, based on similarities in the FBA sequences with the catfish and zebrafish NCCRP-1 peptides.
This new gene family includes several F-box domain-containing proteins and a predicted C. elegans protein.
Received: 20 June 2001 / Accepted: 31 August 2001 |
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Keywords: | : Zebrafish — Nonspecific cytotoxic cells — Antigen-binding domain — Catfish — expressed sequence tags — F-box — F-box-associated domain |
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