| 8mol/L脲中rhIL-3含量的测定 |
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| 引用本文： | 孔祥文,丘文.8mol/L脲中rhIL-3含量的测定[J].生物技术,1997(6). |
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| 作者姓名： | 孔祥文 丘文 |
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| 作者单位： | 西江大学生物系应用生物教研室!广东肇庆，526061(孔祥文)，肇庆天里医学技术开发公司!广东肇庆，526060(丘文) |
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| 摘 要： | 为解决8mol／L脉中rhIL－3的定量问题，以卵清蛋白作内标，进行常规SDS－PAGE后，作激光灰度扫描，并计算rhIL－3和卵清蛋白的峰面积，发现两种蛋白峰面积的比值与rhIL－3浓度在0．2－1．0mg／ml间呈良好线性关系。查标准曲线可以计算出8mol／L脲中rhIL－3的含量。重复性测定表明该法具有较好的重现性。
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| 关 键 词： | rhIL－3 8mol／L脲 SDS—PAGE |
Determination the Concentration of rhIL- 3in 8mol/L Urea Solution |
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| Kong Xiangwen.Determination the Concentration of rhIL- 3in 8mol/L Urea Solution[J].Biotechnology,1997(6). |
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| Authors: | Kong Xiangwen |
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| Abstract: | In order to resolve the problem that how to determine the concentration of rhIL- 3 in 8mol/L urea, the ovalbumin was chosed as internal standard when running SDS-PAGE of rhIL-3.Scanning the SDS-PAGE gel with the Ultroscan XL, there exsits a gdri linear relation betweenthe Peak area ratio of rhIL- 3 and ovalbumin and the concentration of rhIL-3 within 0.2-1.0mg/ml, so it is easy to caculate the concentration of rhIL-3 according to the standard curve ofrhIL-3. And the precision of the method is good with the CV 6. 9- 9. 7%. |
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| Keywords: | rhIL-3 8mol/L Urea SDS-PAGE |
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