Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene |
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| Authors: | Vliegen Inge Jacobs Jan A Beuken Erik Bruggeman Cathrien A Vink Cornelis |
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| Affiliation: | Department of Medical Microbiology, University Hospital Maastricht, Maastricht Infection Center, PO Box 5800, 6202 AZ Maastricht, the Netherlands. |
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| Abstract: | To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h. |
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