Endogenous indole-3-acetic acid and abscisic acid in apple microcuttings in relation to adventitious root formation |
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Authors: | Dominique Noiton John H. Vine Michael G. Mullins |
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Affiliation: | (1) Department of Agronomy and Horticultural Science, The University of Sydney, 2006 Sydney, NSW, Australia;(2) Department of Pharmacy, The University of Sydney, 2006 Sydney, NSW, Australia;(3) Present address: DSIR Fruit and Trees, Private Bag, Havelock North, New Zealand |
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Abstract: | The effects of applying indole-3-butyric acid (IBA) for periods up to 48 h were examined in difficult-to-root microcuttings (from newly-established cultures) and in easy-to-root microcuttings (from long-term subcultures) of Jonathan apple (Malus X domestica Borkh). In easy-to-root material, 20% of the microcuttings produced roots in the absence of IBA, while 6 h exposure to 10 M IBA gave 100% rooting of microcuttings. In contrast, root formation in difficult-to-root material was IBA-dependent. Maximum rooting of these microcuttings (50%) required 24 h exposure to 10 M IBA.Variation in the endogenous levels of free indole-3-acetic acid (IAA) during the course of root induction was similar in microcuttings of both types but there were marked differences in endogenous abscisic acid (ABA) levels. In easy-to-root microcuttings ABA remained at a constant low level, but in difficult-to-root material ABA exhibited marked fluctuations and was present at higher concentrations than in easy-to-root microcuttings. |
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Keywords: | auxin GC-MS-SIM indole-3-butyric acid Malus X domestica rooting |
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