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Plasmid profiles of Lactobacillus plantarum spp. found as contaminants in Hemerocallis plant tissue cultures
Authors:C Leifert  F Berger  GSAB Stewart  WM Waites
Institution:Department of Plant and Soil Science, University of Aberdeen, Aberdeen, UK;Institut für Pflanzenbau und Tierhygiene in den Tropen und Subtropen, Universität Göttingen, Göttingen, Germany;Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, UK
Abstract:Plasmid profiles of 30 strains of Lactobacillus plantarum isolated from 3-year-old tissue cultures and surface sterilized stem sections of glasshouse grown plants of three different varieties of Hemerocallis were compared to pinpoint the source of Lact. plantarum contamination in plant tissue cultures. Since the profiles of strains isolated from in vitro and in vivo plant material were generally identical, it is thought that the source of Lact. plantarum contamination is the plant material used to initiate plant tissue cultures.
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