Displacement chromatography of simple protein mixtures, using carboxymethyldextrans |
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Authors: | Anthony R Torres Elbert A Peterson |
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Institution: | Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205, U.S.A. |
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Abstract: | Simple mixtures of proteins have been used as models to demonstrate that the fractionation of proteins by carboxymethyldextrans on an anion exchanger (Peterson, E.A. (1978) Anal. Biochem. 90, 767--784) is fundamentally a displacement process. Examples include the separation of two closely similar proteins, the A and B forms of beta-lactoglobulin. The use of carboxymethyldextran spacers was essential for good separation, since resolution based on protein--protein displacement was generally inadequate. The origins and effects of heterogeneity in the preparations used as spacers are discussed. |
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Keywords: | protein chromatography displacement carboxymethyldextran |
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