| 用核酸杂交法检查北京地区幼儿园正常儿童尿中人巨细胞病毒DNA |
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| 引用本文： | 王淑凤,马静雅,张礼璧. 用核酸杂交法检查北京地区幼儿园正常儿童尿中人巨细胞病毒DNA[J]. 病毒学报, 1988, 0(1) |
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| 作者姓名： | 王淑凤 马静雅 张礼璧 |
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| 作者单位： | 中国预防医学科学院病毒学研究所 北京(王淑凤，马静雅)，中国预防医学科学院病毒学研究所 北京(张礼璧) |
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| 摘 要： | 以克隆的人巨细胞病毒(Human Cytomegalovirus,简称HCMV)DNA片段做探针,用核酸杂交方法检查了50例北京地区幼儿园正常儿童的尿标本。查出HCMV DMA阳性者24例,占48％。在两个群体和两个年龄组之间,阳性检出率有显著性差异。利用探针可查出同源DNA5～10pg,而不与其它病毒或细胞DNA杂交。标记不同的探针,使用不同的固定方法,其敏感性不同。此法可在分子水平上查出HCMV基因,比分离病毒更迅速敏感。
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| 关 键 词： | 巨细胞病毒 核酸杂交 |
SURVEY OF URINE SPECIMENS FROM KINTER-GARDEN CHILDREN IN BEIJING FOR CYT-OMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION METHOD |
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| Wang Shufeng Ma Jingya Zhang Libi. SURVEY OF URINE SPECIMENS FROM KINTER-GARDEN CHILDREN IN BEIJING FOR CYT-OMEGALOVIRUS INFECTION BY DNA-DNA HYBRIDIZATION METHOD[J]. Chinese journal of virology, 1988, 0(1) |
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| Authors: | Wang Shufeng Ma Jingya Zhang Libi |
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| Abstract: | 50 urine specimens from the kintergarden children in Beijing were exa-mined by using DNA hybridizatioa test with cloned HCMV probe. 24 out of 50 samples shown positive result. The positive rate is 48 per cent.Different positive rates between the 2 kiatergardens and the 2 age groups were observed. The probe can detect homologous DNA at pg Ievel and specifically hybridized with DNA of HCMV but not with other virai or cell DNAs tested.The DNA-DNA hybridization test is more rapid and more sensitive thaa the virus isolation method. The assay will be useful for rapid virai diagn osis and clinical research. |
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| Keywords: | Human Cytomegalovirus Nucleic acid hybridization |
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