A flow cytometric screening test for detergent-resistant surface antigens in monocytes. |
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| Authors: | Zsuzsanna Wolf Evelyn Orsó Tobias Werner Alfred Boettcher Gerd Schmitz |
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| Institution: | Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany. |
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| Abstract: | Rafts resemble cholesterol- and glycosphingolipid-enriched, liquid-ordered plasma membrane microdomains, showing resistance to nonionic detergents, and are involved in various cellular processes. In the present study, we have tested surface antigens on resting and lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes for their detergent resistance (i.e. raft-association), by flow cytometry. Constitutive (CD14, CD32, CD55), or LPS-induced (CD81) raft-association, and detergent solubility (i.e. exclusion of rafts) (CD71) of monocyte antigens in the presence of 0.01% Triton X-100 are clearly demonstrated. Flow cytometric detergent insolubility is a powerful tool for rapid screening the raft-association of monocyte antigens in a whole-blood assay. |
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