| 基因组改组及其在木质纤维素水解液乙醇发酵菌种选育中的应用展望 |
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| 引用本文： | 张作阳,田 沈,孟繁艳,闫 飞,李丰田,杨秀山. 基因组改组及其在木质纤维素水解液乙醇发酵菌种选育中的应用展望[J]. 微生物学通报, 2009, 36(6): 0900-0904 |
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| 作者姓名： | 张作阳 田 沈 孟繁艳 闫 飞 李丰田 杨秀山 |
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| 作者单位： | 首都师范大学,生命科学学院,北京,100048 |
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| 基金项目： | 国家863计划项目(No. 2007AA05Z405); 中青年骨干教师资助项目(No. 07531310499); 北京市优秀人才资助项目(No. 005400704) |
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| 摘 要： | 能高效代谢木质纤维素水解液中的可发酵糖、同时可耐受/分解发酵抑制剂的菌种, 是利用木质纤维素为原料生产燃料乙醇技术的关键。基因组改组技术是近些年发展起来的一项新型育种技术, 该技术已运用于食品和医药行业菌种的改良。本文综述了基因组改组技术的原理、方法、特点、及其运用, 并对其在木质纤维素水解液乙醇发酵菌种选育方面的应用进行了展望。
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| 关 键 词： | 基因组改组 育种 乙醇发酵 木质纤维素 |
Genome Shuffling and Its Prospect for Strain Improvement in Ethanol Production from Lignocellulosic Hydrolysates |
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| ZHANG Zuo-Yang,TIAN Shen,MENG Fan-Yan,YAN Fei,LI Feng-Tian and YANG Xiu-Shan. Genome Shuffling and Its Prospect for Strain Improvement in Ethanol Production from Lignocellulosic Hydrolysates[J]. Microbiology China, 2009, 36(6): 0900-0904 |
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| Authors: | ZHANG Zuo-Yang TIAN Shen MENG Fan-Yan YAN Fei LI Feng-Tian YANG Xiu-Shan |
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| Affiliation: | College of Life Science, Capital Normal University, Beijing 100048, China;College of Life Science, Capital Normal University, Beijing 100048, China;College of Life Science, Capital Normal University, Beijing 100048, China;College of Life Science, Capital Normal University, Beijing 100048, China;College of Life Science, Capital Normal University, Beijing 100048, China;College of Life Science, Capital Normal University, Beijing 100048, China |
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| Abstract: | Commercial production of bioethanol from lignocellulosic hydrolysates requires efficient fermenting strains. The abilities of the strain to converting all types of sugars in the hydrolysate to ethanol in high yield and to effectively tolerating/metabolizing inhibitors are necessary. Genome shuffling is a novel method for breeding, and it has been applied in pharmaceutical and food industry. This review summarized the technique of genome shuffling including principle, process, applications and its prospect for strains improvement in ethanol production from lignocellulosic hydrolysates. |
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| Keywords: | Genome shuffling Breeding Ethanol fermentation Lignocellulose |
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