AMP and adenosine are both ligands for adenosine 2B receptor signaling |
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| Authors: | Jessica K Holien Benjamin Seibt Veena Roberts Evelyn Salvaris Michael W Parker Peter J Cowan Karen M Dwyer |
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| Institution: | 1. ACRF Rational Drug Discovery Centre, St. Vincent’s Institute of Medical Research, Fitzroy, Victoria 3065, Australia;2. Immunology Research Centre, St. Vincent’s Hospital Melbourne, PO Box 2900, Fitzroy, Victoria 3065, Australia;3. Department of Medicine, The University of Melbourne, PO Box 2900, Fitzroy, Victoria 3065, Australia;4. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Fitzroy, Victoria 3010, Australia |
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| Abstract: | Adenosine is considered the canonical ligand for the adenosine 2B receptor (A2BR). A2BR is upregulated following kidney ischemia augmenting post ischemic blood flow and limiting tubular injury. In this context the beneficial effect of A2BR signaling has been attributed to an increase in the pericellular concentration of adenosine. However, following renal ischemia both kidney adenosine monophosphate (AMP) and adenosine levels are substantially increased. Using computational modeling and calcium mobilization assays, we investigated whether AMP could also be a ligand for A2BR.The computational modeling suggested that AMP interacts with more favorable energy to A2BR compared with adenosine. Furthermore, AMPαS, a non-hydrolyzable form of AMP, increased calcium uptake by Chinese hamster ovary (CHO) cells expressing the human A2BR, indicating preferential signaling via the Gq pathway. Therefore, a putative AMP-A2BR interaction is supported by the computational modeling data and the biological results suggest this interaction involves preferential Gq activation. These data provide further insights into the role of purinergic signaling in the pathophysiology of renal IRI. |
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| Keywords: | Adenosine AMP Docking Homology model |
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