Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry |
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Authors: | Giuseppe Caruso Chiara Cavaliere Patrizia Foglia Riccardo Gubbiotti Roberto Samperi Aldo Lagan |
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Institution: | aDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy |
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Abstract: | Drought is an abiotic stress that strongly influences plant growth, development and productivity. By proteomics study it is possible to identify the complex mechanism of water-stress response. The aim of this research was the analysis of wheat (Triticum durum) proteome changes under stress conditions by applying a severe water deficit treatment for 7 days. Stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis in conjunction with matrix-assisted laser desorption ionization-time of flight mass spectrometry. Thirty-six protein spots showed a reproducible significant change between control and stressed samples. The reasonable implications in drought response of the identified proteins were discussed. Results provide new insights that can lead to a better understanding of the molecular basis of drought-sensitivity in plants. Therefore, the obtained data could suggest the development of drought resistant wheat varieties, in order to improve agricultural production in dry regions. |
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Keywords: | Drought Gel electrophoresis MALDI-TOF Proteomics Wheat |
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