Nucleic acid sensor for insecticide detection |
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| Authors: | Solanki Pratima R Prabhakar Nirmal Pandey M K Malhotra B D |
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| Institution: | Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi-110012, India. |
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| Abstract: | Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO(-) (4))-doped PANI film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry. These dsCT-DNA-PANI-ClO(4)/ITO and PANI-ClO(4)/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) measurements. This disposable dsCT-DNA-PANI-ClO(4)/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005 ppm) and trichlorfon (0.01 ppm) in 30 and 60 s, respectively. |
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| Keywords: | nucleic acid sensor electrochemical polyaniline perchlorate cypermethrin trichlorfon |
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