Isolation and characterization of a toxic intracellular protein from Vibrio parahaemolyticus |
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Authors: | E. Umeh A. Njoku-Obi |
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Affiliation: | (1) Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria;(2) Department of Biological Sciences, University of Agriculture, Makurdi, Benue State, Nigeria |
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Abstract: | A toxic factor released from disrupted cells of Vibrio parahaemolyticus was partially purified by gel filtration after precipitation with (NH4)2SO4 at 40% saturation. The factor, which was a thermostable protein of 63 kDa, lysed human erythrocytes at a concentration of 0.15 g ml-1. Its LD50 by intravenous injection into mice was 6.4 g. Fluid accumulated in suckling mice force-fed with the toxic material (1 to 25 g). Haemolytic activity, which occurred maximall at 37°C and pH 7.0 was enhanced by Ca2+, Cu2+ and Zn2+, each at 1 mm. Anti-toxic-factor serum agglutinated V. parahaemolyticus cells. The factor may play a role in the pathogenesis of V. parahaemolyticus infections and in the host's defence mechanisms against infection by the microorganism. |
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Keywords: | Immunogenicity pathogenicity toxic intracellular protein Vibrio parahaemolyticus |
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