Production of High-content Galacto-oligosaccharide by Enzyme Catalysis and Fermentation with Kluyveromyces marxianus |
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Authors: | Chao-Chun Cheng Mei-Ching Yu Tzu-Chien Cheng Dey-Chyi Sheu Kow-Jen Duan Wei-Lun Tai |
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Institution: | (1) Department of Bioengineering, Tatung University, Taipei, 104, Taiwan;(2) Department of Chemical Engineering, Tatung University, Taipei, 104, Taiwan;(3) Department of Chemical Engineering, Northern Taiwan Institute of Science and Technology, Taipei, 112, Taiwan |
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Abstract: | Of three β-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) from lactose, the latter produced the highest
yield of trisaccharides and tetrasaccharides. GOS production was enhanced by mixing β-galactosidase glucose oxidase. The low-content
GOS syrups, produced either by β-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by
Kluyveromyces marxianus, whereby glucose, galactose, lactose and other disaccharides were depleted, resulting in up to 97% and 98% on a dry weight
basis of high-content GOS with the yields of 31% and 32%, respectively.
An erratum to this article can be found at |
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Keywords: | Batch fermentation Galacto-oligosaccharide β -galactosidase Glucose oxidase Kluyveromyces marxianus |
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