Characterisation of maltase from enteropathogenic Escherichia coli |
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| Authors: | O Olusanya PO Olutiola |
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| Institution: | Department of Medical Microbiology and Parasitology, University of Ife, Ile-Ife, Nigeria;Department of Microbiology, University of Ife, Ile-Ife, Nigeria |
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| Abstract: | Abstract Enteropathogenic strains of faecal Escherichia coli produced significantly ( P < 0.01) more maltase than the non-pathogenic strains of the organism. The enzyme was induced by maltose but repressed by glucose and fructose. The maltase was partially purified by ammonium sulphate precipitation, followed by dialysis and gel permeation chromatography. The partially purified maltase had an M r of 144500 and an apparent K m of approx. 7.6 mM for maltose. The enzyme was stimulated by Ca2+, inhibited by Cu2+, Hg2+, Uo2+, IAA and EDTA, and exhibited optimum activity at pH 6.5 at 30°C. |
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| Keywords: | Escherichia coli maltase |
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