PCR扩增泥鳅和大鳞副泥鳅SRY盒基因

以特异扩增人ＳＲＹ基因保守区的一对引物，研究了泥鳅和大鳞副泥鳅基因组中ＳＲＹ盒基因的扩增。结果表明，该引物可以在泥鳅中扩增出四条带，其长度分别为２００，５５０、９４０和１０００ｂｐ。在大鳞副泥鳞中扩增出三条带，大小为２００，５５０和９００ｂｐ。经Ｓｏｕｔｈｅｒｎ杂交显示出二者的阳性带为２００和５５０ｂｐ。阳性带在雌雄个体间和两个物种间无差异。

THE AMPLIFICATION OF SRY|BOX GENES IN THE GENOMIC DNA OF %MISGURNUS AUGUILLICAUDATUS% AND %PARAMISGURNUS DABRYANUS% BY PCR

Using the primers which specially amplify the conservative motif of Human SRY gene,we studied the PCR amplification of SRY box(Sox) genes in genomic DNA of Misgurnus auguillicaudatus and Paramisgurnus dabryanus .The results showed that 4 bands present in the PCR products of Misgurnus auguillicaudatus ,their length was 200,550,940 and 1 000bp.3 bands present in Paramisgurnus dabryanus ,they were 200,500 and 900bp,respectively.Southem blotting indicated that the 200 and 550bp bands were specially positive.There was no difference between male and females,no differences were found between these two species.