Effects of optimal and supra-optimal salinity stress on antioxidative defence,osmolytes and in vitro growth responses in <Emphasis Type="Italic">Sesuvium portulacastrum</Emphasis> L. |
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Authors: | Vinayak H Lokhande Tukaram D Nikam Vikas Y Patade Mahendra L Ahire P Suprasanna |
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Institution: | (1) Department of Botany, University of Pune, Pune, 411 007, India;(2) Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India; |
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Abstract: | The effect of optimal and supra-optimal concentrations (0, 200, 400 or 600 mM) of NaCl on the growth, osmotic adjustment and
antioxidant enzyme defence was studied in the in vitro cultures of Sesuvium portulacastrum. A significant increase in growth, tissue water content (TWC) and fresh to dry weight ratio (FW/DW) was observed in the shoots
exposed to 200 mM salt. Minimum damage to the membrane in terms of low relative electrolytic leakage (REL) and malondialdehyde
(MDA) content and better osmotic adjustment at 200 mM salt stress was coupled with the higher accumulation of sodium ions
and total soluble sugars as against low proline and glycine betaine contents. A fine tuning of antioxidant enzyme activities
(superoxide dismutase, catalase and ascorbate peroxidase) was also found to be responsible for the optimum growth of shoots.
In contrast, sub-optimal (0 mM) and supra-optimal concentrations (400–600 mM) of NaCl significantly affected the growth, water
status and increased the REL as well as MDA content of the shoots due to the accumulation of toxic concentrations of saline
ions. The highest accumulation of proline and glycine betaine in addition to antioxidant enzyme activities exhibited higher
osmotic adjustment and survival of the shoots under sub- or supra-optimal concentrations of NaCl as a penalty to reduced growth. |
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