Use of very low density light fluorescence microscopy: study of real-time of the development of chromatin of unicellular mouse embryos |
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| Authors: | P Debey J P Renard M Geze P Adenot |
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| Institution: | Unité en Développement Concerté I.N.S.E.R.M.-I.N.R.A., Institut de Biologie Physico-Chimique, Paris. |
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| Abstract: | Video enhanced fluorescence microscopy coupled to digitized image processing was used to follow the dynamics of chromatin changes in live one cell mouse embryos. The experimentation on live material was made possible through the use of very low concentrations of the DNA specific fluorophore, Hoechst 33342, and very low irradiation intensities, which maintain a good viability of embryos. |
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