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Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones
Authors:Akashi T  Fukuchi-Mizutani M  Aoki T  Ueyama Y  Yonekura-Sakakibara K  Tanaka Y  Kusumi T  Ayabe S
Affiliation:Department of Applied Biological Science, Nihon University, Kanagawa, Japan.
Abstract:Cytochrome P450 cDNAs, AFNS2 and TFNS5, were isolated from snapdragon and torenia petal cDNA libraries, respectively, based on the sequence homology with licorice CYP93B1 cDNA encoding (2S)-flavanone 2-hydroxylase. They were expressed in yeast and identified to encode flavone synthase II catalyzing direct conversion of flavanones to flavones probably via 2-hydroxyflavanones.
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