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Effects of methanol on the synthesis of aspartame precursor catalysed byPseudomonas aeruginosa elastase |
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| Authors: | K H Lee P M Lee Y S Siaw K Morihara |
| Institution: | (1) Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Pertanian Malaysia, 43400 UPM Serdang Selangor, Malaysia;(2) Department of Chemistry, Indiana University, Shah Alam Campus in Malaysia, ITM/MUCIA, Section 17, 40000 Shan Alam, Selangor, Malaysia;(3) Institute for Applied Life Science, University of East Asia, Ichinomiya-Gakuen cho 2-1, 751 Shimonoseki-shi Yamaguchi, Japan |
| Abstract: | Summary Pseudomonas elastase was found to be efficient in catalysing the reaction betweenN-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester producing the aspartame precursor in aqueous and aqueous methanolic solutions. 25% (v/v) methanol was most favourable for the synthesis where about 100% increase in yield was obtained compared to that in aqueous solution.Abbreviations N-cbz-L-Asp N-benzyloxycarbonyl-L-Aspartic acid - L-Phe-OMe L-phenyl alanine methyl ester - HPLC high performance liquid chromatography. All the % of methanol is a volume % in water unless otherwise specified |
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