在96孔板中进行抗脂质过氧化的微量测定

以Fe²⁺/半胱氨酸诱导大鼠肝微粒体为基本模型,根据硫代巴比妥酸(TBA)反应原理,优化不同反应条件,建立了一种在96孔板上进行抗脂质过氧化测定的一步反应方法，该方法的灵敏度不低于传统的试管法，而且还具有微量、快速、简便等优点，特别适用于大规模筛选和研究抗氧化剂.此外，也可用于其它系统诱导的抗脂质过氧化的测定.

One-Step Microassay for the Measurement of Anti-Lipid Peroxidation in 96-Wells Plate

Based on the mode of formation of microsomal lipid peroxdation induced by Fe²⁺/cysteine and the principle of TBA reaction,a simple one-step microassay for the studying and screening of antioxidants in 96-wells plate was established. The comparative studies between the procedures in 96-wells plate and in test tube were also conducted.This microassay requires only small volume of diluted microsome and tested compounds,having the advantages of reliability,rapidness,simplicity and convenience. It is especially suitable for the pilot screening of antioxidants as well as the simultaneous study for many samples. This method can also be used to measure the lipid peroxidation induced by other systems.