Mechanisms of CO2 fixation in bacterial photosynthesis studied by the carbon isotope fractionation technique |
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| Authors: | R Sirevåg B B Buchanan J A Berry J H Troughton |
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| Institution: | (1) Botanical Laboratory, University of Oslo, Blindern, P.O.B. 1045, Oslo 3, Norway;(2) Present address: Department of Cell Physiology, University of California, Berkeley, CA, USA;(3) Department of Biology, Carnegie Institute of Washington, Stanford, CA, USA;(4) Physics and Engineering Laboratory, Department of Scientific and Industrial Research, Lower Hutt, New Zealand |
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| Abstract: | 1. The carbon isotope discrimination properties of a representative of each of the three types of photosynthetic bacteria Chlorobium thiosulfatophilum, Rhodospirillum rubrum and Chromatium and of the C3-alga Chlamydomonas reinhardii were determined by measuring the ratio of 13CO2 to 12CO2 incorporated during photoautotrophic growth. 2. Chromatium and R. rubrum had isotope selection properties similar to those of C3-plants, whereas Chlorobium was significantly different. 3. The results suggest that Chromatium and R. rubrum assimilate CO2 mainly via ribulose 1,5-diphosphate carboxylase and the associated reactions of the reductive pentose phosphate cycle, whereas Chlorobium utilizes other mechanisms. Such mechanisms would include the ferredoxin-linked carboxylation enzymes and associated reactions of the reductive carboxylic acid cycle.Abbreviations RuDP
ribulose 1,5-disphosphate
- PEP
phosphoenolpyruvate |
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| Keywords: | Photosynthetic bacteria Carbon isotope ratio CO2 fixation Chlorobium thiosulfatophilum Rhodospirillum rubrum Chlamydomonas reinhardii Chromatium |
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