BnFAD2、BnFAD3和BnFATB基因的共干扰对油菜种子脂肪酸组分的影响

甘蓝型油菜是一种重要的油料作物,为了改良其种子脂肪酸组分,提升其经济价值,本研究分析了油菜种子发育时期脂肪酸合成积累模式及BnFAD2、BnFAD3、BnFATB基因的表达规律,认为这3个基因在种子发育中后期(授粉后25d起)的高效表达对油酸合成积累有着重要影响。通过Napin启动子诱导对油菜植株中BnFAD2、BnFAD3、BnFATB基因进行RNAi共干扰抑制,以达到提升油酸含量的目的。试验结果表明,转基因油菜种子中BnFAD2、BnFAD3、BnFATB基因的表达受到强烈抑制,种子中油酸含量由66.76%提升至82.98%,且油脂合成的相关基因同步出现表达上调。

Effect of silencing of BnFAD2, BnFAD3, BnFATB gene on fatty acid component in rapeseed(Brassica napus L.)

Brassica napus L.is an important oil crop.In order to improve fatty acid components in rape seed and enhance its economic value,this study analyzed the synthesis and accumulation mode of fatty acid in seed developmental stages and the expression pattern of BnFAD2,Bn FAD3 and BnFATB genes.The results suggested that BnFAD2,BnFAD3 and BnFATB genes played important roles in the synthesis and accumulation of oleic acid in mid-late period of seed development stage (25 days after pollination) with its efficient expression.Through Napin promoter induction,BnFAD2,BnFAD3 and BnFATB genes were suppressed by RNA interference technology to enhance the oleic acid content.The results showed that the BnFAD2,BnFAD3 and BnFATB genes' expression was strongly inhibited in transgenic oilseed and the oleic acid content increased from 66.76％ to 82.98％.At the same time,the expression of related genes up-regulated synchronously.