Potentiometric and NMR studies on Cd coordination with the histidine-containing Ac184-188NH2 prion protein fragment |
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Authors: | Valeria Guantieri Alfonso Venzo Mario Acampora |
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Affiliation: | a Dipartimento di Scienze Chimiche, Via Marzolo 1, 35131 Padova, Italy b CNR, Istituto di Scienze e Tecnologie Molecolari, Via Marzolo 1, 35131 Padova, Italy c CNR, Istituto di Chimica Biomolecolare, Via Marzolo 1, 35131 Padova, Italy |
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Abstract: | To elucidate the specific mode and site of binding between metal ions and prion protein (PrPc), we synthesized the pentapeptide Ac184-188NH2 (AcIKQHTNH2), corresponding to helical region II of the protein, and its analogous acetylated at the lysine side chain. The acid-base properties of both peptides and their interaction with Cd2+ were studied in aqueous solution by NMR and potentiometry. Speciation data were compared with those achieved for Cd2+/4-methylimidazole, taken as the reference system. Both NMR and potentiometric data indicate that Cd2+ is coordinated by the histidine residue. The involvement of the side chain amine of lysine in the metal coordination is excluded by NMR data, whereas a role for either the carbonyl or the amide group of threonine is suggested. |
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Keywords: | Prion protein Histidine Cadmium complexes Cd2+ coordination NMR Potentiometry |
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