Occurrence and metabolic role of the pyruvate dehydrogenase complex in the lower termite Coptotermes formosanus (Shiraki) |
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| Institution: | 1. Global Research Laboratory, College of Pharmacy, Pusan National University, Busan 46241, South Korea;2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center, Sapporo, Japan;3. Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;4. Department of Microbiology, Pusan National University, Busan 46241, South Korea;1. Institute for Integrative Biology of the Cell, UMR9198, CNRS, Université Paris-Sud, CEA, Gif-sur-Yvette, France;2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center, Sapporo, Japan;3. Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;4. National Institute for Basic Biology (NIBB), Okazaki, Japan;5. Department of Biology, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany;1. Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA;2. Institute of Biological Sciences, Donghua University, Songjiang, Shanghai 310029, China;3. Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA;4. Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, USA;1. Department of Biochemistry, Universidade Federal de São Paulo, Escola Paulista de Medicina, 04044-020 São Paulo, SP, Brazil;2. Department of Biochemistry, Instituto de Química, Universidade Federal do Rio de Janeiro, 21941-909 Rio de Janeiro, RJ, Brazil;3. Department of Parasitology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil;4. Centro de Pesquisas René Rachou, FIOCRUZ, 30190-002 Belo Horizonte, MG, Brazil |
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| Abstract: | The activities of the pyruvate dehydrogenase complex in extracts of the gutted body, head, foregut/midgut and hindgut (hindgut epithelium and microorganisms) tissues of the lower termite Coptotermes formosanus (Shiraki) were determined by measuring the 14C]-acetyl-CoA produced from 2-14C]-pyruvate and the 14CO2 produced from 1-14C]-pyruvate. The activities of pyruvate dehydrogenase, l-lactate dehydrogenase, acetyl-CoA synthetase, malate dehydrogenase (decarboxylating), and acetate kinase in the termite tissues and the hindgut also were determined. The sum (7.1 nmol/termite/h) of the pyruvate dehydrogenase complex activities in the termite tissues other than the hindgut was five times higher than the activity in the hindgut. Significant amounts of l-lactate dehydrogenase activity were found in all of the tissues. All of the tissues other than the hindgut had significant amounts of acetyl-CoA synthetase activity. Malate dehydrogenase (decarboxylating) activity was about ten times higher in the hindgut extract than in the gutted body and head extracts and the activity in the foregut/midgut extract was very low. These results indicate that acetyl-CoA for the TCA cycle is produced effectively in the tissues of the termite itself, both from pyruvate by the pyruvate dehydrogenase complex and from acetate by acetyl-CoA synthetase. |
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