Growth modulation of human tumor cells by a growth-inhibiting activity derived from tumorigenic V79 chinese hamster cells |
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Authors: | Ichiro Ichinose Shuji Nakano Taito Esaki Tatsuhiko Koga Kenji Mitsugi Hidenori Yamada Yoshiyuki Niho |
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Institution: | (1) First Department of Internal Medicine, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi Higashi-Ku, Fukuoka, 812 Fukuoka, Japan;(2) First Department of Internal Medicine, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi Higashi-Ku, Fukuoka, 812 Fukuoka, Japan |
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Abstract: | Summary A growth-inhibiting activity was identified in supernatants of the neoplastic V79 Chinese hamster cell line based on its ability
to inhibit the proliferation of the same cell line. The partially purified activity, provisionally termed “growth inhibiting
factor” (GIF) activity, inhibited the growth of a wide variety of human tumor cells, but not various normal human fibroblasts.
This species-nonspecific activity was reversible, saturable, and highly potent in tumorigenic cell lines, and was noted in
both monolayer culture and in soft agar. The inhibitory activity CIF was also exhibited in a chemically defined serum-free
medium supplemented with insulin and transferrin. GIF activity was stable to acid, heat, trypsin, and dithiothreitol but sensitive
to alpha-chymotrypsin. The pattern of growth modulation by GIF on V79 cells was apparently different from those exhibited
by bifunctional peptides such as transforming growth factor-beta, tumor necrosis factor-alpha, and interleukin-1-alpha. In
addition, GIF activity cannot be ascribed to these cytokines based on the physicochemical and immunologic properties. Although
GIF has yet to be purified to homogeneity, these data suggest that GIF might be a novel growth regulator which has a critical
role in regulating growth of V79 cells. The growth modulation of tumor cells by this tumor-derived growth inhibiting activity
suggested the presence of an autocrine growth regulatory mechanism even in tumor cells. |
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Keywords: | tumor-derived inhibitor density-inhibition autocrine growth regulator |
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