Inhibition of geranylgeranyl diphosphate synthesis in in vitro systems |
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Authors: | Andrea Steiger Ursula Mitzka-Schnabel Werner Rau Júrgen Soll Wolfhart Rüdiger |
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Affiliation: | Botanisches Institut der Universität München, Menzinger Str. 67, 8000 München 19, W. Germany |
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Abstract: | The incorporation of [14C]mevalonate and [14C]isopentenyl diphosphate into geranylgeranyl diphosphate was investigated in in vitro systems from Cucurbita pepo (pumpkin) endosperm and from Avena sativa etioplasts. Mevalonate incorporation was effectively inhibited in the pumpkin system by geranylgeranyl diphosphate and geranylgeranyl monophosphate but less effectively by phytyl diphosphate or inorganic diphosphate. Membrane lipids, geranyllinalool, or lecithin enhanced mevalonate incorporation in the Cucurbita system. Incorporation of isopentenyl diphosphate was also enhanced by lecithin and inhibited by geranylgeranyl diphosphate in the Cucurbita system. No lipid enhancement was found in the Avena system; inhibition by GGPP required a much higher GGPP concentration than in the Cucurbita system. |
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Keywords: | etiolated seedlings endosperm mevalonic acid isopentenyl diphosphate. GGPP, geranylgeranyl diphosphate PhPP, phytyl diphosphate FPP, farnesyl diphosphate MVA, mevalonic acid IPP, isopentenyl diphosphate DMAPP, dimethylallyl diphosphate GPP, geranyl diphosphate GL, geranyllinallool Hepes, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid AMO 1618, 2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidin-carboxylate-methylchloride |
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