Pentacyclic triterpenoids and sterols from seven species of mangrove |
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Authors: | Amitabha Ghosh Suniti Misra Ashis K. Dutta A. Choudhury |
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Affiliation: | 1. Marine and Estuarine Biological Research Unit, Department of Marine Science, University of Calcutta, 35 B.C. Road, Calcutta 700 019, India |
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Abstract: | The isolation of pentacyclic triterpenoids from seven species of fresh mangrove leaves using a simple and rapid method is described. The leaves were homogenized using chloroform—methanol and the extract was diluted with water to precipitate out triterpenoids which were separated into neutral and acidic fractions. These were analysed by gas-liquid chromatography as acetyl and trimethylsilyl ether derivatives on a 3% OV-17 column. Sterols were isolated from the chloroform layer by preparative thin layer chromatography and were analysed by gas-liquid chromatography as their trimethylsilyl ether derivatives on a 3% OV-17 column. The triterpenoids found were α-amyrin, β-amyrin, lupeol, oleanolic acid and ursolic acid in most of the samples. Sterols found in all the samples were cholesterol, campesterol, stigmasterol, sitosterol and stigmast-7-en-3β-ol. Retention indices of the triterpenoids and sterols have been determined. |
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Keywords: | Acanthaceae Rhizophoraceae Rhizophoraceae Fabaceae Rhizophoraceae Chenopodiaceae pentacyclic triterpenoids sterols. |
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