Mitogenic effect of estradiol on MCF-7 human breast cancer cells can be modulated by serum |
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| Authors: | A Najid A Nicolas M Tixier G Habrioux |
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| Affiliation: | Laboratoire de Biochimie, Faculté de Pharmacie, Université de Limoges, France. |
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| Abstract: | The MCF-7 human breast cancer cell line responds to estradiol stimulation in vitro by increased proliferation only if prolonged subcultures in dextran-coated charcoal-treated fetal calf serum have been made previously. This growth stimulation is not obtained when cells are grown in medium containing 5% untreated fetal calf serum. We describe here the culture conditions under which we obtain a reproducible estradiol effect on cell growth. |
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