Hepatic lipid metabolism: effect of spermine, albumin, and Z protein on microsomal lipid formation. |
| |
Authors: | S C Jamdar |
| |
Institution: | Departments of Internal Medicine and Biochemistry, Medical College of Virginia, Richmond, Virginia 23298 U.S.A. |
| |
Abstract: | Effects of spermine, bovine serum albumin, and Z protein on microsomal lipid formation from sn-glycerol 3-phosphate and 14C]palmitoyl CoA were investigated. In the presence of these agents, microsomal lipid formation was stimulated. This was attributed to the activation of sn-glycerol 3-phosphate acyltransferase and to the inhibition of palmitoyl CoA hydrolase. In addition to palmitoyl CoA, spermine also reacted with microsomal membranes in causing their aggregation, and ATP reversed the effect of spermine. Further studies indicated that the interaction of spermine with palmitoyl CoA, rather than with microsomal membranes, was responsible for the activation of glycerolipid formation or to the inhibition of palmitoyl CoA reductase. Examination of the intravesicular distribution of sn-glycerol 3-phosphate acyltransferase and palmitoyl CoA hydrolase and the effects of structural integrity of microsomal vesicles on these two membrane-bound enzymes indicated that the activation of glycerolipid formation and the inhibition of palmitoyl CoA hydrolase by spermine, bovine serum albumin, or Z protein may be closely linked with the structural integrity of microsomal vesicles. |
| |
Keywords: | Direct all correspondence to author at present address: S C Jamdar Medical Research Institute Florida Institute of Technology Melbourne Florida 32901 |
本文献已被 ScienceDirect 等数据库收录! |
|