DDGGE, a simplified denaturing gradient gel electrophoresis method for the detection of sequence variations in PCR fragments |
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| Authors: | Susanne Logemann Mike SM Jetten |
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| Institution: | (1) Kluyver Institute for Biotechnology, Delft University of Technology, Julianalaan, Delft, The-Netherlands |
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| Abstract: | The need for rapid analysis of sequence variations in PCR fragments of the same length is increasing in medical diagnostics and environmental studies. Therefore a modified denaturing gradient gel electrophoresis (DGGE) method was developed in which mixed PCR fragments of 1,500 bp could be analysed on a conventional DNA sequencing gel apparatus. In addition, PCR primers without long GC-clamps could be used to amplify the target genes. © Rapid Science Ltd. 1998 |
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