Anther culture of some perennial triticeae |
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Authors: | J E Marburger R R -C Wang |
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Institution: | (1) USDA-ARS, Forest and Range Research Laboratory, Utah State University, 84322-6300 Logan, UT, USA;(2) Research Geneticists, USDA-Agricultural Research Service, 84322-6300 Logan, UT, USA |
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Abstract: | Three field grown Agropyron spp. (crested wheatgrasses) and two Thinopyrum spp. (intermediate and tall wheatgrasses) were evaluated for anther culture response. Hormonally modified potato extract and 85D12 media induced pollen embryogenesis. Modified Murashige and Skoog media were tested for their effects on callus proliferation and plantlet regeneration. Callus induction frequency and plantlet production were highest (25.0% and 45.8%, respectively) for Thinopyrum ponticum (2N=70) (tall wheatgrass). One-hundred and nine albino plantlets were produced from T. ponticum Jose both by direct regeneration on 85D12 medium and through a callus phase from potato extract media. This is the first report of plantlet production from anther culture of a Triticeae perennial forage grass. Further experimentation with environmental and cultural conditions may result in the production of green plantlets.Abbreviations MS
Murashige and Skoog (1962) medium
- NAA
naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- 2-ip
2-isopentenyladenosine
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid
Cooperative investigations of the USDA-Agricultural Experiment Station and the Utah Agricultural Experiment Station, Logan, UT 84322. Approved as Journal Paper No. 3596 |
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