| Abstract: | On increasing aeration rate in chemostat cultures of E. coli the redox potential (Eh) of the culture changes from ?l50 mv to +250 mv. During this period only a slight increase in measured dissolved oxygen concentration is observed. It is suggested that Eh be used as a control variable for aeration in the oxygen limited growth condition, whilst dissolved oxygen concentration be used as control variable in the presence of excess oxygen. Change in cytochrome b1, three tricarboxylic acid cycle enzymes and hydrogenase can be related to culture Eh. These changes are discussed. |
