Effect of vitamin B6 deficiency on pancreatic acpinar cell function |
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Authors: | M Singh |
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Institution: | Gastrointestinal Research Laboratories V.A. Medical Center (FHD) Augusta, Georgia 30904, USA |
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Abstract: | The present study was designed to determine the effect of vitamin B6 deficiency on pancreatic acinar cell function. Rats were either fed or rendered B6-deficient by a purified B6-deficient diet; half of the latter being replenished with IP pyridoxine before sacrifice. Body weight, pancreatic weight, RNA and DNA content were decreased in B6-deficient animals. These changes were considered to be due to inanition resulting from decreased food intake. Amylase content of pancreas in B6-deficient animals was less compared with B6-replenished animals. Although slightly higher in B6-deficient animals, the incorporation of L-phenylalanine14C into total tissue proteins was not significantly different in the three groups of animals. On B6-replenishment, incorporation of L-phenylalanine14C into nascent proteins was diminished in spite of higher tissue amylase and protein content. Vitamin B6 deficiency decreased total RNA content and adenine-8-14C incorporation into RNA. DNA content was diminished but incorporation of thymidine-2-14C into DNA was increased. On replenishment with B6, thymidine-2-14C incorporation decreased significantly compared to control animals. Secretion of amylase was diminished commensurate with decreased content. It is concluded from these studies that B6-deficiency induced DNA injury, decreased RNA turnover and increased protein turnover resulting in diminished amylase content. These data indicate that B6-deficiency so frequently encountered in alcoholism may contribute to the pancreatic injury in this clinical condition. |
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