Monoclonal antibodies against pig transierrin. Blocking and binding activity |
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| Authors: | I. Trebichavský B. Macků V. Viklický M. Koukal P. Šiman |
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| Affiliation: | (1) Institute of Microbiology, Hungary;(2) Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 14220 Prague 4 |
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| Abstract: | Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin bound to specific receptors on the plasma membrane. The binding of iodinated pig transferrin to PK cells was studied. It could be blocked by nonlabelled transferrin in excess, by pig serum or by anti-pig transferrin monoclonal antibodies. PTF-03 expressed the lowest blocking activity among the antibodies tested. |
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