Cambial tissue culture and subsequent shoot regeneration from mature black locust (Robinia pseudoacacia L.)

Callus initiation and subsequent shoot regeneration were obtained from cambial tissue of mature black locust trees. Shoot regeneration was highly tree specific, and was only achieved when calli derived from a particular genotype (tree #1) were transferred to medium containing 6-benzylaminopurine. The studies described here show that the immediate use of a mature genotype as starting material for tissue cultural manipulation is possible by using cambial explants with the proper in vitro conditions. We also found that stems could be stored at 4°C for 8 months prior to culture initiation, with no decline in the viability of the cultures. Tissue sources can thus be continuously available.Abbreviations BAP 6-benzylaminopurine - NAA 1-naphthyleneacetic acid - IBA indolebutyric acid