Effect of gonadotropin-releasing hormone on estrogen receptor messenger ribonucleic acid level in perifused pituitary cells

Summary 1. We examined the potential effect of GnRH pulses on pituitary estrogen receptor mRNA level.2. The treatment of perifused pituitary cell aggregates with four hourly pulses of GnRH (10 nM/1 min/h) resulted in a marked increase in the steady-state level of ER mRNA (25%vs unstimulated control, n = 3).3. No changes were observed for the LH ß mRNA. Data suggest, for the first time, that a cross-talk between the GnRH and nuclear ER may occur in the gonadotrope cells.