Detection of a soluble serum antigen by a new monoclonal antibody ED8 in patients with breast cancer |
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| Authors: | M Mortada G Pancino C Charpin M H Schlageter F Calvo A Roseto |
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| Affiliation: | Division d'Immunocytologie appliquée, Université de Compiègne, Centre Benjamin Franklin. |
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| Abstract: | A murine hybridoma, secreting monoclonal antibody ED8, was generated by immunization with the breast cancer cell line H466B. ED8 reacts with a 300 kDa antigen present on H466B cell surface. The antigen can be detected in serum from breast cancer patients, using ELISA and immunoblotting. |
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