应用荧光原位杂交技术检测人类APPSWE基因在转基因小鼠染色体上的定位及位置效应

应用荧光原位杂交技术研究了人类淀粉样前体蛋白基因瑞典型突变（APPSWE）在转基因小鼠首建、F1及F2代小鼠染色体上的整合及定位，结果在2只首建转基因小鼠中，分别观察80个分裂相，出现杂交信号的核型分别为34及36个，检出率为42.5%和45%；1只F1及1只F2代转基因小鼠中，分别观察100个分裂相，出现杂交信号的核型分别为33及30个，检出率为33%和30%。转基因分别整合在8号、1号、17号和2号染色体上，提示转基因APPSWE已稳定整合到转基因小鼠的染色体上，并通过生殖细胞遗传给予子代，证实转基因在小鼠染色上的整合可能是随机的多点整合，同时，对不同整合位点的转基因小鼠进行了表型研究，结果发现不同整合位点对表型具明显影响。

Mice by Means of in situ Fluorescence Hybridization

In situ fluorescence hybridization (FISH) was used to study the integration and localization of human APP_SWE gene on chromosomes of transgenic mice. It was found that in 2 founder mice, 34 and 36 metaphases of 80 metaphases observed had shown obvious specific hybridizing signals, the detection rates were 42.5% and 45%, respectively; meanwhile, in 1 F1 and 1 F2 mice, 33 and 30 metaphases out of 100 metaphases observed had specific hybridizing signals, the detection rates were 33% and 30%, respectively. The transgenes were localized on chromosome 8, 1, 17 and 2. The results indicated that transgene APP_SWE were integrated stably into the chromosomes of transgenic mice and could be transmitted to offsprings through germ cells. The transgenes were randomly integrated into multiple sites of mice chromosomes. Meanwhile, the phenotypes of transgenic mice were also studied and different integrated sites had obvious effects on the pheonotypes.