Studies on oxygen and volume restriction in cultured cardiac cell: possible rearrangement of sarcolemmal lipid moieties during anoxia and ischemia-like states |
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Authors: | Ramesh Vemuril Marcel Mersel Michael Heller Arié Pinson |
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Institution: | (1) Laboratory for Myocardial Research, Institute of Biochemistry, the Hebrew University — Hadassah Medical School, P.O.B. 1172, 91-010 Jerusalem, Israel;(2) Centre-de Neurochimie, 5 Rue Blaise Pascal, 67084 Strasbourg Cedex, France |
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Abstract: | Summary Cultured heart cells have been shown useful for investigating states of oxygen and volume restrictions, simulating anoxia and ischemia-like states at cellular levels. The sarcolemma has been implicated as one of the early sites of ischemic damage; therefore, lactoperoxidase catalyzed radioiodination was used to study accessibility of the sarcolemmal lipid moieties to this enzymatic probe, reflecting their exposure to the extracellular environment, hence the biophysical state of the sarcolemma. These studies have shown that within one hour of ischemic injuries: (1) The degree of labelling in the total phospholipid fraction is consideraly increased; and (2) Profound changes in the relative extent of labelling of different phospholipid classes were observed. The PE/PC labelling ratio increases dramatically with the progress of ischemia-like state. We suggest that early during ischemic injury, reorganization of the cell surface phospholipids occurs and discuss possible relations to the energy charge of the cell. |
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Keywords: | ischemia cultured heart cells sarcolemma phospholipids anoxia |
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