Control of 3T3 cell proliferation by calcium |
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Authors: | A L Boynton J F Whitfield R J Isaacs H J Morton |
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Institution: | (1) Division of Biological Sciences, National Research Council of Canada, K1A OR6 Ottawa, Canada |
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Abstract: | Summary When a population of 3T3 mouse cells was subcultured regularly at confluency, the original epitheliodid or stellate cells
disappeared and, by the ninth passage, they had been replaced by spindle-shaped cells. The original cells proliferated only
when the extracellular calcium concentration exceeded 0.1mm, and their proliferative activity became maximum only when the calcium concentration was 0.5mm. The spindle-shaped cells were much more sensitive to proliferative stimulation by calcium. Although these cells also could
not proliferate without extracellular ionic calcium, they proliferated maximally in the presence of as little as 0.05mm calcium. Thus, calcium is a major regulator of the proliferation of 3T3 mouse cells. Moreover, it appears that the sensitivity
of the proliferative machinery to the calcium ion can vary greatly within an established cell line. |
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